429
126316470016778155
Maryam90211520234177315473555678855
Sulaiman181511520234962463544925447218581019
Mohammed28921152023417734048862580297910881256
Al420711520234137246
Saadi45111152023496233371540592
Department9021366014981156232313348384495569643679
of16181366014975112
Biology1766136601495372147167242323383
College9021510014989164183203277358433
of13721510014975111
Sciences15201510014954123143217291360434478
Dissertation9011826017098132193255344389431524566599689773
Title90120210122426696120184
THE17902023012043111164
EXPRESSION19822023012054115174235288338388410497571
PATTERN25812023012059133176218272333407
OF30152023012087136
DEATH31792023012074127201244312
ASSOCIATED35192023012074124174261342365439481535609
PROTEIN41562023012059120207249303325399
KINASE4583202301205982156230280333
149442023012055
(DAPK1)50272023012037111185245304359396
IN5451202301202296
NORMAL55752023012074161221313387434
DORSAL60362023012075162222272346392
ROOT17902143012061148234277
GANGLION20952143012087161235322368391478552
NEURONS26752143012074127193253340414464
AND31672143012074148222
FOLLOWING34172143012048135181228315411433507594
PERIPHERAL40392143012059113174196255324377438512558
NERVE46252143012074127188258312
INJURY4965214301202296145210271330
Faculty9012296012248114178238262292350
Advisor12792296012274140196220264328360
Dr.17902298012074104125
Rasheed19432298012060129167228293358427
Al2398229801207494
Hammadi25192298012069137231324393461481
Defense9012451012270134162226286330394
Date13232451012270136166230
1217902453012055111
January19292453012048116177238306336390
201523462453012056111167222
Abstract9012766012274140184214246312376406
Death-associated1790277209660112166193242269323354385438490506560587639694
protein2506277209676100152179231247296
kinase1282427720966177125180211263307
(DAPK1)3154277209650109168216263307337
is351327720963768
a3603277209675
calcium/calmodulin3701277209672127143194210259334369421475491566619674722738754803
(Ca2+/CaM)4526277209650116170214263298363418491521
regulated506927720964596150199215269297349403
ser-ine/threonine5494277209652104128155171220272307334383407458511559575624676
kinase.619227720966177126181212264286
Increasing179028680961867119143194249280296345399
body2215286809655107162205
of244228680965782
evidence2547286809656100116171223272324376
supports294528680963684139193246270297328
the329528680963281133
significance345028680963551105154170190209261315364416468
of394028680965782
DAPK14049286809660119166213258
protein433328680965579131158210226275
in463028680962170
cancer4722286809656111160211263287
and5036286809655104158
CNS5221286809665124164
diseases.541228680965571102154208239291323345
The577928680963987139
role59412868096288096148
of611128680965782
DAPK16220286809660119166214258
in179029640961665
peripheral1877296409677129153169223272324348403419
nerve2318296409671123147192244
regeneration258429640964698152204253305329383410426479528
and3134296409677125180
neuropathic3336296409671123172196248303357385433449501
pain3859296409677132148197
remains407829640964698173228244292323
com-pletely4424296409674126201228282298350377429445488
unexplored.4934296409671120172210265281333358409464486
We5442296409699148
aimed561229640967793168220275
to5909296409649102
investigate603329640963887131183214241257311366393445
DAPK11790306009660119166213258
expression2070306009667105160184235266297313366415
pattern2507306009669124151178230254304
along283430600966985137186240
with309630600968298125173
key3292306009655107149
pro-346430600966993145172
and3658306009669118173
anti-apoptotic3853306009669118145161188243297349404431484511527578
cell4454306009666118134150
signaling462630600964662116165219235251300354
molecules5017306009675127143195247296312364395
(p53,543430600964499143188210
Bax,5666306009661115154176
AKT,5864306009674121155169
ERK5,6055306009658107154198220
P38)6298306009662106150180
and1790315609655103158
to197031560963386
verify2078315609650102126142167210
the231031560963382134
possibilities2466315609660113144175191245261277293320336388420
of290831560965883
DAPK1-NMDA3019315609660119166213258284343417477536
NR2B3583315609659107152198
relationship38033156096308197152179195247296327376392447
in427231560962270
dorsal4365315609660113137168222238
root462531560963082135162
ganglion4809315609660114163217233249301350
neurons5181315609655107155180232280311
(DRG)551531560963594143213242
at577931560966188
25889315609650
hours,5961315609655107156180211233
76216315609651
days6289315609660115158189
and1790325209655103158
14197032520965297
days2089325209662117160191
following230232520963386102118170236252301355
sciatic267932520963991107161188204256
nerve2957325209657109133177229
injury.32093252096237288137161204226
ATF3345732520966795133178
was3665325209666121152
used383932520965687139194
as405532520966394
neuronal4171325209657109157181233282337353
injury45463252096247289137161204
marker.4780325209675130154194246270286
Using508932520966091107156209
gene5321325209661113162214
expression555732520966098153177228260291307359408
analysis5987325209662111166182225256272303
and6312325209662111166
im-munohistochemistry179033480961691118193241290342391407438465518570618670745761792820844887
assessed269933480966697128180211242294348
the307033480963887139
effects323133480966388114166217244275
of352933480966388
nerve3640334809659111136180232
injury.38943348096277692141165208230
The414633480964594146
results431433480963687118167183210241
showed457733480964391144210262317
that491633480963887142169
DAPK15107334809671130177225269
mRNA5409334809675124183242
was5673334809678133164
ex-pressed5859334809663102128183207258289321373427
and6308334809666115170
translated179034440962751106155186202256283335390
to220234440963486
functional231034440963280129181208224276325380396
protein272834440966185137164216232281
in303134440962271
normal3124344409655107132209263279
DRG3432344409659108178
neurons.3632344409655107155180232280311334
Soon398834440964698151199
after421034440966085113165189
sciatic442134440963789105159187203254
nerve4697344409656108132176228
injury49473444096227187136160203
(2517234440963680
hours),5274344409655108156180211241263
DAPK15565344409660119167214258
was5852344409666121152
significantly602634440963753107156172191211262317366393409452
(p<0.05,179035400963084133177199243288310
2.2212235400964467111
fold)22553540096257894149178
up-regulated2455354009649104130154206260308324379406458513
in299035400961665
the307735400962776128
injured32273540096176582130154206261
L4351035400963781
and3614354009654103158
L5379435400963781
DRG3898354009659108178
compared4098354009652104179234288312364419
with453935400966783110159
contralateral4720354009652104153180204259275329356408432487503
uninjured524535400964998114163179227252303358
side.562535400963147102154176
However,5846354009655107173225270322346360
76228354009644
days6294354009655110153184
after179036360965580107159183
axotomy199936360965593146173225300343
a2368363609655
profound244936360965479131156208257306360
decrease2836363609654106158182234288320372
was3234363609666121152
observed3412363609652107138190214258310365
in379936360962069
DAPK13894363609660119166214258
level,417436360962072117169185207
with440736360966682109158
further458736360963078102129178230254
reduction486336360962880135184235262278331380
that526536360963180134161
reached544936360962879134186235287341
its58123636096204879
minimum591736360967591140156231279354
level629436360962072116168184
at179037320965582
14189437320965094
days2010373209661115158189
postoperatively.2221373209660113144171223278330354409436452496548564607629
In287237320962473
addition,2967373209660115170186213229281330352
at334137320966188
73451373209650
days3523373209660115158189
after373437320966085112164188
injury,39453732096217086135159202224
most4197373209675127158185
of440437320965883
DAPK14515373209660119166214258
positive4795373209660112144160187203247299
injured51163732096227087135159211266
neurons5404373209654106155179231280311
were5743373209666118142194
ATF3596537320965986125170
positive,6157373209660112143159186202247299321
while1790382809666115131147199
after201138280967197124176200
142233382809661105
days2360382809671126169200
this258238280964392108139
correlation2743382809669121145169221237291318334387436
was3201382809683137168
not3391382809666118145
observed3558382809669124155207231275327382
as3962382809671102
DAPK14086382809676135183230274
immunoreactivity4382382809633108183231280333357408463515542558602618645688
decreased5092382809672124175199251306337389444
in555838280963281
injured56613828096328197146170222277
ATF35960382809675103142186
positive6168382809671123154170198214258310
neurons.1790392409649101149174226274305328
Interestingly,214039240963079106158182233264292308356410426469491
DAPK1,2653392409672131179226270292
p532967392409667111156
and3145392409666115170
Bax3337392409658113151
exhibited3510392409664103152168222238265317372
almost390439240966783158210241268
same419539240964397172224
expression4442392409664102157181232263294310363412
pattern4876392409666121148175227251302
in520039240962877
axotomized5299392409666105157184237312328362414469
lumbar579039240962876151206261285
DRG.6097392409671120190212
The633139240964695147
results179040200962476107155171199230
also204240200966177108160
revealed222440200963183127179234250302356
that260340200963382137164
sciatic278940200963789105160187203255
nerve3066402009655107131176228
injury33164020096227187136160203
had3541402009655110165
no3728402009655107
effects385840200965883109161212239270
on4151402009658107
the428040200963483135
gene4437402009660112161213
expression467240200965997152176227258289305358407
of510140200965984
ERK5,520740200964998145189212
P38544140200965498142
and5605402009661110165
AKT5799402009659106140
at596140200966189
all60724020096617793
studied618740200963865113168184236291
time179041160962743118170
points.1983411609655107123172199230252
Moreover,2258411609674126150202254298350375388
NMDA2669411609659133192252
NR2B2944411609659107152198
mRNA3165411609675123182242
expression342941160965291145169221252283299352400
increased385241160961665117141193247278330385
at426041160965582
74364411609645
days4432411609655110152183
and4638411609655104159
contin-ued4820411609651104153180196245271320372427
to526941160962880
up-regulate5372411609648103130154205259308324378406458
significantly585241160963248101150166186205257312360387403446
until632141160964998125141157
14179042120964489
days1901421209655110153184
postoperatively2108421209654107138165217272324348402430446490542558601
(p<0.05,273142120963085133178200244288310
3.6306442120964567111
fold).31974212096267894149179201
In342042120961968
a3510421209655
contrast,3588421209652104153180204259290317339
our3950421209652101125
immu-nofluorescence409742120961792167215242291343362382431483507559590641693742794846
results496542120962576108156172199230
showed521742120963281133200252307
a5546421209655
decrease5624421209655107158183234289320372
in601842120961766
its61064212096164374
protein620342120965579131158210226275
level179043080961668112164180
in199243080962170
DRG2089430809659108178
neurons2289430809653105154178230279310
during2625430809655104128144193247
this28944308096318096127
time304343080963248123175
period;3245430809655107131147199254276
however,3543430809654106172224269321345359
a3928430809655
strong40054308096366387139188242
positive4273430809655107138154181197242294
NMDA4594430809659132192251
NR2B4872430809659108152198
immunoreactivety509243080962196171219268320345396451503530546590642669712
appeared5831430809654109164216270294346401
in625443080962169
the634543080963281133
satellite179044040963186113165181197213240292
cells2104440409652104120136167
that229344040962876131158
surround247344040963180104128180229278333
injured28284404096166581129154205260
large-sized31104404096167195149201228259275309361415
neurons3548440409648100149173225274305
in387544040961665
L4396244040963782
and4066440409655104158
L5424644040963882
DRG4350440409660108178
neurons.4550440409649101150174226275306328
In490044040961867
addition,4989440409655110165181208224276325347
immunofluorescence535844040961691166215264316336355404456480532563615667715767819
double6200440409654107155210226278
labeling179045000961671125177193209258312
revealed212445000963183127179234250302357
that250345000963483138165
DAPK12697450009660119166213258
and2977450009662110165
NMDA3172450009659132192251
NR2B3452450009660108152198
are367245000966286138
co-localized3832450009659112138154206258313329345379431486
in434045000962372
normal4434450009656108132210264280
and4736450009662111166
injured49244500096237288137161213267
DRG5221450009659108178
neurons.5421450009656108156180232281312334
In577845000962574
conclusion,5874450009659111160212228276307323376425447
the634345000963483135
down-regulations1790459609655107174222249273325379427443498525541593642673
of248845960965278
DAPK12590459609660119167214258
following28704596096288096112165231247296350
sciatic324245960963486102156183199251
nerve3515459609652104128172224
injury37614596096196884133157200
along398645960965470123171225
with423645960966783110158
other441945960965380129181205
vital46464596096476390145161
pro-apoptotic483145960965579131158212267319374401453481497548
players540445960965571125168220244275
promoting570445960965579131206258285301350404
neuronal6130459609652104152176228277332348
survival179046920963180104148164208263279
might208846920967591145193221
shed233146920962776128183
light25364692096122882131158
on2713469209652101
the283646920962472124
mechanisms2979469209675127179228282331347378453484
of348246920965277
peripheral3578469209654106131147201250302326381397
nerve3993469209649101125169221
regeneration.423746920962072126178226278302357384400453501524
We4779469209677125
also492346920965470101154
suggest509946920962876130184236267294
that541546920962473127154
NMDA5588469209659133192251
might585846920967591145194221
modulate6097469209675128183231247302329381
neuropathic1790478809649101149174226280335362411427478
pain2291478809660114130179
through249247880963382106158207261309
satellite282447880963691118170186202218245297
cell3143478809658110126142
but3307478809660109136
not3465478809655107134
neurons3621478809655107155179231280311
73954478809650
and4027478809660109164
14421347880965094
days4329478809661115158189
after454047880966186113165189
PNS4751478809653113152
injury.49264788096217086135159202224