516
2162046470016778155
Sunitha747614023496246406458579727865
Pulikkot167061402341062563644165817468881006
Department747828014981156232313348384495569643679
of1463828014975112
Anatomy1611828014983157238274349460520
College747972014989164183203277358433
of1216972014975112
Medicine13659720149110184264284353372446520
and1922972014981155235
Health2194972014973148229248284354
Sciences2585972014954123142217290359433478
Dissertation7451418017098132193255344389431524566599689773
Title74516120122426696120184
ESTABLISHMENT17761614012054103146220277324346396464556610684726
OF25301614012087136
A26931614012074
3D27951614012055130
CULTURE29521614012082147193236302362416
MODEL33951614012092179254307354
OF37761614012087136
GASTRIC39391614012087162211254315337419
STEM4385161401205093146238
CELLS46511614012081135181227277
SUPPORTING49561614012049115174233320381423446520607
THEIR55911614012043111164187248
DIFFERENTIATION586716140120749714519324730817761734012054128170193267309332419493
INTO2297173401202296139226
MUCOUS25501734012092158239326392441
CELLS30191734012081135181227277
USING33241734012065115138212299
MICROFIBROUS36511734012092114195256343391414471532619685734
POLYCAPROLACTONE4413173401205914619225233340746652761466073481585894510191072
SCAFFOLD55131734012050131205254302389435510
Faculty7451887012248114178238262292350
Advisor11231887012274140196220264328360
Prof1776188901205989154186
Sherif19891889012050111176206226258
Karam22741889012060128158226320
(CMHS),26221889012037118210278328365393
Dr30421889012075105
Yaser31741889012060119157222252
Greish34541889012087117182202241302
(COS),37841889012036118205254291319
Prof4131188901205989154185
Abdel-Hamid43441889012074142211276296329397466560579648
Mourad50201889012092157218248316385
(COE)54321889012037119206259296
Defense7452042012270134162226286330394
Date11672042012270136166230
1317762044012055111
May19152044012091160213
201521562044012055111166222
Abstract7452197012274140184214246312376406
In177622040961867
the186522040962776128
stomach,201522040963158110185240292341363
epithelial2400220409652106122149198250266282337353
stem277522040963158110185
cells2982220409652104120136167
are317122040965479130
responsible332322040962476107162214263294310364380432
for377722040962578102
glandular390122040965470124173228277293347371
homeostasis4294220409649102177229281312339394425441472
and4788220409654103158
perpetual4968220409655107131185237264313368384
production537422040965478130185234286313329381430
of582622040965277
four592622040962477125150
main6097220409676130146195
cell1776230009652104120136
lineages19342300096244089141195249301332
secreting228823000963991143167219246262311365
mucus,2683230009675123175224255277
acid,2982230009663115131185208
pepsinogen3212230009662114169200216265317371423472
and3706230009662111166
hormones.3894230009657109133211263312364395417
These433323000964291143174226
stem458123000963966118193
cells4797230009659111127143174
could4993230009660113161177232
represent524723000963284138163214245297346373
a5642230009663
source572723000963991140164215267
for601723000963385109
cell6148230009660112128144
therapy177623960962776128152207261304
or210223960966286
tissue22102396096375384115164216
engineering2448239609662110164180229281333357373422476
in294623960962574
cases3042239609662116147199230
of329523960966287
gastric3404239609663118149176200216268
mucosal3704239609675123175227259313329
damage.4055239609665119194249303355377
The445423960964493145
aims462123960966480155186
of482923960966287
this493923960963685101132
study509323960964168117171214
were5339239609667119143194
1)555523960965484
to566123960963789
manufacture5782239609675130178227252307358386434458510
various177624920964499123139191240271
forms20692492096217398175206
of229324920965277
scaffolds239224920962779133159184236252307338
using27482492096498096145198
polycaprolactone,2964249209655107123166218272327351403419474526553605654706728
2)371024920964474
to380624920962375
test390324920962375106133
the405824920962372124
suitability42042492096277591118173228244260276303346
of456824920965277
these466724920962372124155207
scaffolds489624920962778133158184236252307338
for52562492096217397
growth537124920965478130197224272
of566124920965378
mouse5757249209675127176207259
gastric6033249209654109140167191207259
stem177625880963158110185
(mGS)1983258809635110180220250
cells,2255258809657109125141172194
and2471258809661109164
3)265725880965080
to275925880963385
evaluate2866258809658102157173221276303355
whether3249258809666115167194243295319
this35902588096338298129
culture3741258809657106122149198222273
system403725880963679110137189264
could4323258809658110159175230
tolerate457525880963285101153177231259311
exposure490825880965796150203234282306358
to528825880963385
acidic5395258809661112128183199251
environment5668258809658106151167191243292367419468495
for618525880963183107
possible1776268409655107138169185240256308
future210626840962573100149173225
applications.2353268409654109163179195247302329345397446477499
Three287526840963382106158210
forms310726840962577101179210
of333926840965277
polycaprolactone3438268409654107123166217272327351403419474525552605654706
scaffold416626840963082137162187240256311
were4498268409667119143195
fabricated:471526840962579134158174226280307359414436
nonporous,5173268409649102150205257281333382413435
microporous563026840967591143167219274326350402451482
and6134268409654103158
microfibrous.177627800967591143167219238258312337389437468490
Microscopic229427800967389141165217248300352407423475
examination279127800965795150225241290344371387440489
and3307278009654103158
mechanical3492278009675127179228282331347399454470
testing398427800963284115142158207261
revealed426727800962981125177232248300354
some464427800963688163215
similarities488127800963652127143159214238254281297349380
between5288278009655107134201253305353
the566427800963281133
microfibrous582427800967591142167219238258312336388437468
scaffold177628760963183137163188240256311
and2109287609654103158
extracellular228828760965291118142196248300316332381397451475
matrix2785287609675130157181197235
of304228760965277
the314128760962775127
stomach329028760963158110185240292340
wall.3652287609666121137153175
Examination384928760964281135210226275330357373425474
of434528760965277
mGS4444287609675144184
cells4650287609651103119135166
cultured4838287609652100116143192216268322
on5182287609652101
different53042876096557196122174198249298325
forms565128760962577101179210
of588228760965277
scaffold598228760963082136161187239255310
revealed177629720962476120172227243295350
their214829720963583135151175
preferential234629720966286138163215239290339366382437453
growth282129720966286138204231280
on3123297209660109
microfibrous326229720967591143167219239258313337389437468
scaffolds375329720963890145170195248264318349
with413229720966783110159
an4313297209662111
initial44462972096247389116132186202
increase467129720962372124148200254285337
in503129720962372
cell5125297209660112128144
number5291297209656105180235287311
followed562429720963385101117170236288343
by5989297209662105
their611629720963584136152176
differentiation17763068096557196121173197249298325341396423439491540
into233830680962775103155
gland251530680966480135184239
mucous2786306809675124176228277308
cells3116306809662114130146177
after331630680966590117169193
93531306809655
days.3608306809666120163194216
Various3847306809666113137153205254285
mechanical,4165306809675127178227282331347398453469491
chemical,4678306809663112164239255306361377399
morphological5110306809675127151206255307323376429445497552568
and5700306809665114169
molecular5901306809675128144196248296312367391
examinations177631640965290145220236285340367383435484515
revealed231331640961870114166221237289343
that267831640962170125152
this28523164096217086117
3D2985316409644104
culture3104316409652101117144193217268
system33943164096256899126178253
could3663316409652104153169224
partially3903316409654109133160176231247263306
tolerate42313164096217389141165220247299
amoderate4546316409655146198253305329383411463
acidic5024316409655107123178194245
environment.5285316409652101145161186238286361413462489512
Interestingly,58193164096116087139163215246273289338392408451473
only1776326009652101117160
after195832600965580107159183
3-day216332600964471125180223
culture2408326009652100116143192216268
at269832600965482
pH2801326009655110
5.5,293332600964466110133
mGS3087326009675145185
cells3294326009652104120136167
differentiated34833260096557196121173197249298325341395422474529
into40343260096166592144
mucous4200326009675124176228277308
cells4530326009651103119135167
as471832600965586
demonstrated4826326009655107182234283314341365420447499554
by540232600965497
binding552132600965571119174190239293
of583632600965277
N-acetylD-glucosamine-specific593532600965986141192244272314330177633560966086140156205256309340394469485534586613644698750802818838857909
Griffonia2707335609688112128153179231280296350
simplicifolia308033560964965140194210226278294319372388404458
II356033560963755
lectin363733560963486138165181230
and3889335609673121176
significant408733560965066119168184204223275330378406
up-regulation4515335609666121148172223277326342396424440492541
of507833560967096
Spdef5196335609658112167219244
mRNA5462335609693142201260
expression.5745335609670108163187238269300316369418440
In620733560963685
conclusion,1776345209652104153205221269300316369418440
a2243345209654
3D2319345209650109
culture2450345209657105121149197221273
model2750345209675127182234250
of302234520965783
mGS3132345209675144184
cells3338345209657109125141172
supporting353234520963685139194246270297313362416
their397034520963281133149173
differentiation41703452096557196121173197249298325341395422438491540
into47323452096216997149
mucous4908345209675124175228276307
cells5238345209656108124140171
has5431345209654109140
been5598345209654106158207
established.582734520965788115170224240256287336388443465
This17763548096348399130
study192835480963360109164207
provides215935480965478131175191246298329
the251035480962978130
basis2664354809655109140156187
for287335480962880104
future299935480962776103152176228
use325135480964880132
of340735480965277
stem350635480963361113188
cells3718354809652104120136167
in390735480961867
gastric3998354809654108139167191207258
tissue42793548096294576107155207
engineering4511354809652100154170219271323347363412466
for499935480962779104
regenerative512535480962677131183232284308363390406450502
therapy564935480963078130154209264307
of598035480965277
some607935480963386161213
stomach177636440963158111186240292341
diseases213936440966177108160214245297328
involving249036440962170115167183227243292346
mucosal2864364409675124175228259314330
damage.3216364409660115190245299351373
Keywords:17763834097501011472112622883413762152383609622
stem219638360963158110186
cell,2404383609651103119135158
gastric2584383609654108139166190206258
mucosa,2864383609675124176228259314336
cell3222383609652104120136
differentiation,33803836096557196121173197249298325341396423439491540562
tissue39643836096274374106154206
engineering4192383609652101155171220272324348364413466
Research7024042017081171232322414459549633
Relevance13744042017081171204294372465549638728
and21414042017093177269
Potential24494042017079168210300384426459552585
Impact30744042017039170263355445487
Since70242600964056105156208
stomach93342600963461113188243295344
inflammation12994260096196887107161236311366393409462510
leading183242600961971125180196245299
to215342600963083
ulcers225842600965268120172196227
and2510426009655104158
tumors269142600963079154206230261
are297842600965478130
common3134426009651104179254306355
health3511426009652104159175202251
problems378742600965579131185201253329360
which4172426009666115131183232
require442642600962779134182198222274
new4722426009652104171
therapeutic491542600963079131155210264316365392408460
modalities,5400426009675128183237253269296312364395418
this58404260096307995126
study598842600963462110165208
will62214260096678399115
provide70243560965579131175191246298
the102243560962977129
basis1175435609655109140156187
for138543560962679103
future151043560962675102151175227
use176043560964980132
of191643560965277
stem201543560963360112187
cells2226435609651103119135166
and2416435609655104158
microfibrous259843560967591143167219238258313337389437468
scaffolds308843560963385139165190242258313344
in345443560961867
tissue35434356096284475107155207
engineering3774435609652101154170219271323347363412466
for426243560962779103
regenerative438743560962678131183232284308363390406450502
thera-py491243560962877129153208234289332
for526643560962679103
some539143560963385160212
stomach562543560963360112187242294342
diseases.599143560965571102154209240292323345
Relevant7024502017081171204294372465549591
Publications13324502017091175267301334424516558592681765827
•702472009648
Pulikkot79847200965098114130170211263290
P,111047200965063
Greish119847200966993145161192241
YE,146147200965093115
Mourad1601472009674126175199253308
AH,1934472009659114136
Karam2092472009650105129184259
SM2373472009642116
(2014)251147200963277121165210239
Establishment277247200964677104159213229245277325400452501528
of332547200965378
a3428472009654
three-dimensional350447200963079103155207233288304379431480511527580628683699
culture4228472009652100116143192216268
system451847200963477108135187262
of480547200965277
gastric4907472009654109140167191207259
stem518847200963461113188
cells5401472009651103119135166
supporting559047200963382137191244268295311360413
mucous6028472009675124176228277308
cell702481609652104120136
differentiation8654816096557196121173197249298325341395423439491540
using142748160965384100149203
microfibrous165748160967591143167219239258313337389437468
polycaprolactone2153481609654107123165217272326350403419473525552604653705
scaffolds.288048160963688143168193246262316347370
Cell3277481609665117133149
Proliferation,344848160965276128144160185237261316343359412460483
47:553-63.395348160964993115160204248275319364386
•702491209648
Pulikkot79849120965098114130171211263290
S,111049120964365
Greish120049120967094146162193241
YE,146449120965093115
Mourad1604491209673126174198253308
A-HI,193749120965985140158180
Thomas214049120963685138213267298
SA,2460491209643102124
and2609491209655104159
Karam2790491209650104129183258
SM3070491209643117
(2014)320949120963276121165209239
Evaluation347049120964690145161209264291307359408
of390349120965378
3D4003491209647107
culture4135491209651100116143192216267
model4427491209675128183235251
of470349120965277
gastric4805491209654108139167191207258
stem508649120963361113188
cells5299491209651103119135166
for548749120962881105
tissue56144912096304677108157209
engineering.5848491209652100154170219271323347363412466488
Proceedings70250080964771123175227279334350399453484
of121250080965277
the131250080963179131
8th146650080964875124
Dubai1616500809659108163217233
International18715008096227198150174225279307323375424478494
Conference2392500809665117166191243267319367419471
for288550080962982106
Medical3017500809673125180196248303319
Sciences,335850080964495111163212264316347369
Dubai,3753500809660108163218234256
UAE.4035500809652111154177
•702510409648
Pulikkot79751040964998114130170210263290
S,110951040964264
Saseedharan119551040964297128180232287336390414469518
P,173551040965062
Greish182151040967094146162193241
YE,208551040964992114
Mourad2223510409674126175199254309
A,255651040965981
Karam2659510409650104129183258
SM2939510409642116
(2014)307751040963276120165209239
Growth334051040967094146212239288
and3652510409655104159
Differentiation383551040966076101126178202254303330346400427443496545
of440451040965277
Gastric4506510409670124155182206222274
Stem480251040964269121197
Cells5023510409665117133149180
on5227510409653101
Biodegradable535151040964864116171223277301355410465519535587
Scaffolds.596151040964293148173198251267322353375
Proceedings70252000964771123175227279334350399453484
of121152000965277
the131052000963079131
3nd146352000964796151
Biotechnology163652000964965117144196248297346398414466520563
World2224520009677125150166220
Congress,2469520009665118167220244296327358380
Dubai,2874520009660108163218234256
UAE.3155520009652111154176
•702529609648
Pulikkot79152960964493109125165205257284
S,109452960964062
Greish117552960966993145161192241
YE,143452960964891113
Karam1565529609648102126181256
SM1840529609640113
(2012)197552960962670115159203233
Fabrication222752960963893148172188239294321337390438
of268452960965277
nanofibrous2780529609649103152205224244298322374423454
scaffold325652960962879134159184237253308
for35865296096217498
gastric3702529609654109140167191207259
epithelial3979529609652107123150199251267283338354
tissue43555296096233970101150202
engineering.4576529609652100154170219271323347363412466488
Proceedings510552960964367119171223275330346395449480
of560352960965378
the570352960962372124
International58495296096156491143167217272299315367416471487
Workshop702539209677126150190221270322377
on1096539209652101
Advanced1214539209659114159213262314366421
Material,1652539209673128155207231247302318340
Ras2009539209649103134
El-Khaimah,21615392096435985132181236252327381430452
UAE.2631539209652111154176
Career70255380170109202246336426470
Aspirations121255380170103165257291336428470504593677739
This7025792096348399130
research85457920962476107159214238289338
inspires12145792096166596150166190242273
Sunitha150957920964089137153181229284
to181557920962780
continue1917579209652104153180196245293345
her2284579209649101125
career2432579209651106130181233258
as271257920965485
a2820579209654
post-doctoral2896579209655107138165192247299351378431455509525
fellow34435792096267894110162228
and3694579209654103158
then387457920962776128177
to407357920962779
become4175579209654106158210285337
an4534579209655104
independent466057920961665120172226278327382434483510
stem519257920963158110185
cell5399579209652104120136
scien-tist7025888096318399151200226253269300327
and1052588809654103158
an1232588809655103
academician.1358588809654106161215267342358410426481530552